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Microscope:
Inverted Lecia DMI 6000
Motorized XYZ stage
Andor DSD2 Confocal Module

Camera specification
Camera Andor Zyla 5.5 sCMOS
native resolution 2560x2160
pixel bitdepth 12/16 bit; pixel size 6.5umx6.5um
max frame rate 650f/s(128x128) bypass mode
10x Dry
N PLAN 10x/0.4 dry
20x Dry / 20x Imm
N PLAN L 20x/0.40 dry CORR / HC PL APO CS2 20x/0.70 Imm
40x Dry
HCX PL APO 40x/0.75 dry
63x Oil
HCX PL APO 63x/1.40 oill
Fluorescent filters
A4 (abs.360(+/-)40nm em.470(+/-)40nm) DAPI Hoeschst
I3 (abs.450-490nm em. LONG PASS 515) FITC TRITC
L5 (abs. 480(+/-)40nm em.527(+/-)30nm) FITC
N.21abs.515-560nm em. LONG PASS 590nm) TRITC
Y5 (abs.620(+/-)60nm em.700(+/-)75nm) CY5
Fluorescent filters
DAPI Hoeschst (abs.390(+/-)40nm, 405 DM, em.452(+/-)45nm)
GFP FITC (abs.483(+/-)28nm, 488 DM em.525(+/-)45nm)
TRITC ALEXA568 (abs.556(+/-)20nm, 561 DM, em.609(+/-)54nm)
Cy5, Alexa 647 (abs640(+/-)14mmn, 635 DM, 676(+/-)29nm)
DSD2
Confocality (PSF): 3 sectors provide 1.2 μm, 1.0 μm, 0.8 μm (FWHM
Max frame rates: 22 fps (16-bit, confocal mode, full frame, 1x1 binning)
Minimum exposure: 20 ms (1 full disk rotation)
Fast Aquisition

Fast TileScan

ANDOR DSD2