- Specifiaction
- Objectives
- DMI6000 Filters
- DSD2 Confocal units
- Application
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Microscope:
Inverted Lecia DMI 6000
Motorized XYZ stage
Andor DSD2 Confocal Module
Motorized XYZ stage
Andor DSD2 Confocal Module
Camera specification
Camera Andor Zyla 5.5 sCMOS
native resolution 2560x2160
pixel bitdepth 12/16 bit; pixel size 6.5umx6.5um
max frame rate 650f/s(128x128) bypass mode
native resolution 2560x2160
pixel bitdepth 12/16 bit; pixel size 6.5umx6.5um
max frame rate 650f/s(128x128) bypass mode
10x Dry
N PLAN 10x/0.4 dry
20x Dry / 20x Imm
N PLAN L 20x/0.40 dry CORR / HC PL APO CS2 20x/0.70 Imm
40x Dry
HCX PL APO 40x/0.75 dry
63x Oil
HCX PL APO 63x/1.40 oill
Fluorescent filters
A4 (abs.360(+/-)40nm em.470(+/-)40nm) DAPI Hoeschst
I3 (abs.450-490nm em. LONG PASS 515) FITC TRITC
L5 (abs. 480(+/-)40nm em.527(+/-)30nm) FITC
N.21abs.515-560nm em. LONG PASS 590nm) TRITC
Y5 (abs.620(+/-)60nm em.700(+/-)75nm) CY5
I3 (abs.450-490nm em. LONG PASS 515) FITC TRITC
L5 (abs. 480(+/-)40nm em.527(+/-)30nm) FITC
N.21abs.515-560nm em. LONG PASS 590nm) TRITC
Y5 (abs.620(+/-)60nm em.700(+/-)75nm) CY5
Fluorescent filters
DAPI Hoeschst (abs.390(+/-)40nm, 405 DM, em.452(+/-)45nm)
GFP FITC (abs.483(+/-)28nm, 488 DM em.525(+/-)45nm)
TRITC ALEXA568 (abs.556(+/-)20nm, 561 DM, em.609(+/-)54nm)
Cy5, Alexa 647 (abs640(+/-)14mmn, 635 DM, 676(+/-)29nm)
GFP FITC (abs.483(+/-)28nm, 488 DM em.525(+/-)45nm)
TRITC ALEXA568 (abs.556(+/-)20nm, 561 DM, em.609(+/-)54nm)
Cy5, Alexa 647 (abs640(+/-)14mmn, 635 DM, 676(+/-)29nm)
DSD2
Confocality (PSF): 3 sectors provide 1.2 μm, 1.0 μm, 0.8 μm (FWHM
Max frame rates: 22 fps (16-bit, confocal mode, full frame, 1x1 binning)
Minimum exposure: 20 ms (1 full disk rotation)
Max frame rates: 22 fps (16-bit, confocal mode, full frame, 1x1 binning)
Minimum exposure: 20 ms (1 full disk rotation)
ANDOR DSD2